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Wham clusters all the candidate breakpoints and rounds their positions to the nearest tenth foundation pair. The position with the best go through aid is reported. In case the comfortable-clipped consensus sequence is usually aligned for the putative breakpoint area utilizing the Smith-Waterman algorithm, the breakpoint is additional refined to The
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The soft-clipped sequences that overhang the breakpoint are collapsed into a consensus sequence utilizing a a number of sequence alignment (MSA) offered during the seqAn library [27]. Wham applies 3 filters towards the consensus sequences. Breakpoints are certainly not described in instances the place consensus sequences are shorter than ten bp or
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